IFA Platforms


Fully automated IIF Slide Processing and Reading System

UNIQO is the new high-performance automated system for IFA procedure, from slide preparation to test validation. It provides laboratories with an all-in-one solution which integrates automatic IFA protocol with slide mounting, image acquisition, analysis and archiving.

UNIQO supports standardization eliminating the inconsistency of human intervention and maximizing the accuracy of the overall IFA process, from dispensing and accurate slide mounting, to a more objective images acquisition and analysis.

An innovative software manages and controls the entire experimental workflow guaranteeing optimal slides processing and image capture for a fully automated analysis of immunofluorescence signals and efficient data management.

Moreover, the integrated virtual microscope permits the navigation and the revision of the digitized slide from remote workstations, eliminating the problem of slide storage and fluorescent signal conservation along time.


And more... UNIQO is:

  • Up to 18 slides per run per workstation
  • Up to 160 patients per run per workstation
  • Up to 264 predilutions cuvettes
  • Image acquisition speed: about 30 sec/well
  • Cost and time saving
  • Open system, it allows almost any commercial slide to be processed
  • Accommodates standard 25x75 mm slides, and 24x60 mm coverslips
  • Automatic coverslip mounting without imperfections
  • Lids are automatically placed to preserve slides along the procedure
  • Digitization of the slide: excellent image capture at high emission even at low magnification
  • Fully traceable processing (barcode reading of sample and slides)
  • Full well digitization and archiving
  • Virtual microscope for remote retrieval, reviewing and second opinion
  • Walkaway process with continuous access
  • Automatic titer determination

Reference literature

A Fully Automated Platform Capable of Processing, Scanning and Analyzing IFA Substrates

John Breneman, Sarah Paul, Jeri Seiki, Roger Walker

Clinical Immunology Division, Bio-Rad Laboratories, 4000 Alfred Nobel Drive, Hercules, California 94510

Presented at the 13th Dresden Symposium on Autoantibodies – Dresden, Germany – September 27 – 30, 2017

IFA Substrate Processing, Scanning and Analysis on the IFx 360 System, a Fully Automated Platform

John Breneman, Sarah Paul, Jeri Seiki, Cindy Liedstrand

Clinical Immunology Division, Bio-Rad Laboratories, 4000 Alfred Nobel Drive, Hercules, California 94547

Zenit-PRO, a fully automated indirect immunofluorescence analyser


Martina Fabris, Daria Franceschi, Daria Picchioni, Elio Tonutti, Nicola Bizzaro

On behalf of the Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy - Institute of Clinical Pathology and Immunopathology and Allergy, University Hospital of Udine, Italy - A. Menarini Diagnostics, Firenze, Italy - VISIA Imaging Srl, San Giovanni Valdarno, Firenze, Italy - Chairman, Study Group on Autoimmune Diseases, SIPMeL, Italy


Next-generation IFA testing: teaching an old dog new tricks

Jake Morrow

October 2018

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